Fiber Photometry System
Plug-and-Play Fiber Photometry
At Neurophotometrics, we’re not looking to sell you a bunch of parts. We don’t want you to spend unnecessary time building a system, which in our experience can take 3-6 months and still not produce a very polished user experience. Our system is fully functional as soon as it arrives (provided you’ve got the animals and they’re expressing the right indicators, of course).
Launching Summer 2020
FP3002: The Second Generation
With the sensitivity to pick up the activity of a single cell and the bandwidth to record from multiple freely moving animals and/or brain regions simultaneously, the original FP3001 set the standard for fiber photometry recordings. Through our work with over 100 labs worldwide, we have designed the second-generation system — the FP3002 — to include many upgrades based on feedback from those labs while maintaining the quality standard set by the original model.
The FP3002 has three excitation wavelengths for fiber photometry recording:
- 470 nm for imaging of green calcium indicators (e.g. GCaMP)
- 560 nm for imaging of red-shifted calcium indicators (e.g. RCaMP)
- 415 nm for an interleaved isosbestic control to eliminate motion artifacts (i.e. calcium-independent signals)
The added functionality of the FP3002 includes:
- Real-time processing and flexible control with a new computer interface
- Live data processing of over 100 channels
- Fully redesigned magnetic patch cord mounting system
- Ultra-stable scientific LEDs with patent-pending fiber optic feedback mechanism
- Upgraded sCMOS camera and knife’s edge image splitter for increased sensitivity
- Micro-second precision with synchronization across multiple devices
- Ability to collect analytical measurements for accurate across-subject comparisons
- Integrated optogenetics stimulation system with function generator
The FP3002 can be outfitted with up to two lasers (450nm, 635nm) for optogenetic stimulation. The first laser is free! Include both lasers for an additional customization fee.
Our system is fully compatible with Bonsai, a free and open source program. The data output takes the form of a .csv file, which you can then analyze however you’re most comfortable.
Fiber Optic Cannulae
We offer fiber optic cannulae (FOC) with black ceramic ferrules that have been optimized for fiber photometry with an 80% efficiency cutoff. Be sure to opt for a low autofluorescence fiber and match the numerical aperture (we can help with this).
Ferrule diameter: 1.25mm or 2.5mm
Fiber core diameter: 200um or 400um
FOC are $20/each, with discounts for bulk orders.
$18/each for 100+ FOC
$17/each for 200+ FOC
$15/each for 500+ FOC
FOC are sold in packs of 20. To connect to a patch cord, you will need an adapter. We sell white ceramic split sleeves for this purpose, which are available for $1/each.
Record from up to 12 brain regions using a single implant with our multi-fiber arrays. These 12×1 implants can be cut to custom sizes depending on your experimental needs.
Arrays can be ordered à la carte (minimum 5) or as part of a multi-fiber array starter pack, which includes 5 multi-fiber arrays and a patch cord optimized for the implant.
Onsite Training and Installations
The Neurophotometrics team is committed to providing a top-notch user experience. We want you able to start collecting data as fast as possible, which is why we offer onsite training and installation. Our fiber photometry system may be plug-and-play, but we can help to shorten the learning curve. Our scientists will travel to your lab and train your team over the course of a two-day installation workshop. Plus, the support doesn’t end when our team departs — we’re always available to help, from designing experiments and analysis pipelines to troubleshooting.
We offer special service contracts for core facilities. Inquire with us to learn more.
What Else Do You Need?
Patch Cords: Currently, we only manufacture patch cords for our multi-fiber arrays. For all other applications, we strongly endorse those made by Doric Lenses.
Camera for Behavior Tracking: If you require behavior tracking for your experiments, you’ll need a camera. No need to break the bank on something fancy — we recommend ordering this one on Amazon.